Background:

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Applications:

WB, IF

Name of antibody:

PLCG1 (phospho-Tyr771)

Immunogen:

Synthetic peptide of human PLCG1 (phospho-Tyr771)

Full name:

phospholipase C, gamma 1 (phospho-Tyr771)

Synonyms：

PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1

SwissProt:

P19174

WB Predicted band size:

155 kDa

WB Positive control:

NIH/3T3 cells untreated or treated with serum

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200