Background:

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.

Applications:

ELISA

Name of antibody:

FITC

Immunogen:

KLH conjugated FITC

Full name:

Fluorescein isothiocyanate

CAS No.:

27072-45-3

ELISA Recommended dilution:

2000-4000